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Invitrogen™ Bovine IL-6 Uncoated ELISA Kit

Product Code. 10495335
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Quantity:
5 x 96 Tests
Unit Size:
1 set
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For Research Use Only. All usage must comply with product instructions.

Product Code. 10495335

Brand: Invitrogen™ ESS0029

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562.14 EUR valid until 2025-12-19
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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

ELISA

The Bovine IL-6 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of bovine IL-6. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P26892
Assay Range 78.1 to 5000 pg/mL
Assay Sensitivity <78.1 pg/mL
Description Bovine IL-6 ELISA Reagent Kit
Conjugate HRP
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Product Type ELISA
Sample Type Serum, Supernatant
Specificity No significant cross-reaction with recombinant bovine IL-1ß, IL-2, IL-4, IL-8, IFNg, or TNFa
Sufficient For 5 x 96 microplate wells
Target Species Bovine
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 280826
Gene Symbol IL6
Kit Contents Coating Antibody, Lyophilized Recombinant Standard, Detection Antibody, Streptavidin-HRP, Substrate Solution, Ready to use Stop Solution, 0.16M Sulfuric Acid, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Label or Dye HRP
Measurement Absorbance at 450 nm minus absorbance (at 550 nm)
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 100 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target IL-6
Test Time 24 hrs
Total Assay Time 2 hrs 50 mins
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