BD Fixable Viability Stain 450

Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion of the violet dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells. Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their fluorescence intensity 10-20 fold over that of viable cells. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeablization and subsequent intracellular staining while maintaining stable FVS450 fluorescence.

• Excited by the violet laser (with an excitation maximum of 406nm) and has a fluorescence emission maximum of 450nm
• Contains a dye that reacts with and covalently binds to cell surface and intracellular amines