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STEMCELL Technologies ArciTect™ Human CRISPR Optimization Kits (PE)

Product Code. 17167631
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Product Code. 17167631 Supplier STEMCELL Technologies Supplier No. 1000471

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Description

Complete kit for optimization of genome editing in human cells

  • The ArciTect™ Human CRISPR Optimization Kit is a flow cytometry-based kit designed to enable rapid optimization of genome editing in human cells
  • It can also be used as a positive control for experiments using the ArciTect™ CRISPR-Cas9 genome editing system in human cells
  • The kit comprises ArciTect™ Human B2M sgRNA, ArciTect™ Cas9 Nuclease, and a fluorophore-conjugated beta-2 microglobulin (B2M) antibody
  • ArciTect™ Cas9 Nuclease must first be complexed with ArciTect™ Human B2M sgRNA, followed by delivery into human cells and subsequent culture for 48 - 96 hours
  • Following culture, editing efficiency can be assessed quickly by collection and processing for flow cytometry using a fluorophore-conjugated B2M antibody
  • This kit has been tested and validated for use with the ArciTect™ line of genome editing products
  • By targeting the B2M gene that encodes a ubiquitously expressed cell-surface protein, editing efficiency can be rapidly and quantitatively assessed using flow cytometry methods
  • This technique also enables additional evaluation of editing success such as viability monitoring and/or assessment of cell type-specific marker expression

Specifications

Cell Type Hematopoietic Stem and Progenitor Cells; Intestinal Cells; Pluripotent Stem Cells; T Cells
Description Ab Conjugate: PE
Quantity 1 Kit
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