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Applied Biosystems™ SYBR™ Green Universal Master Mix
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Quantity:
1 x 1 mL
1 x 5 mL
2 x 5 mL
5 x 5 mL
10 x 5 mL
1 x 50 mL
Unit Size:
1 set
5 ampoules
Each
Description
Includes
2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components.
Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
- AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
- dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
- Proprietary buffer enhancements ensure performance and reliability
- Premixed components stored at 2° to 8°C significantly reduce assay setup time
Maximum Flexibility and Convenience:
- Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
- SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
- The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
- Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
- This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
- SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
- Store at -20°C
Order Info
Shipping Condition: Wet Ice
Spécification
Spécification
| Concentration | 2X |
| Content And Storage | The 10 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 2000 reactions based on a 50 μl reaction volume. Store at -20°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Detection Method | SYBR |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Label or Dye | SYBR Green |
| Afficher plus |
Sécurité et traitement
Identifiant produit
- Green PCR Master Mix
- Warning
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362-Take off contaminated clothing.
- MIXTURE LIST-Contains : Tris (hydroxymethyl)aminomethane, Sodium azide
For Research Use Only. Not for use in diagnostic procedures.
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