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Applied Biosystems™ Protein Thermal Shift™ Starter Kit

Product Code. 13473079 Shop All Applied Biosystems Products
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Quantity:
1,000 reactions
Unit Size:
100µg
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Product Code. Quantity Unit Size
13473079 1,000 reactions 100µg
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For Research Use Only. All usage must comply with product instructions.
Product Code. 13473079 Supplier Applied Biosystems™ Supplier No. 4462263
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For Research Use Only. All usage must comply with product instructions.

Description

This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

Applied Biosystems™ Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest.

Save Time and Money with Sample Screening: This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

Two Kits to Choose From: The Protein Thermal Shift™ Dye Kit contains the dye and buffer for performing protein thermal shift experiments, enough for up to 1000 reactions. The Protein Thermal Shift™ Starter Kit contains the Dye Kit plus a control protein and control ligand for test experiments and troubleshooting. The Protein Thermal Shift™ Starter Kit components are packaged in two boxes, one to be stored at -20°C and the other at 4°C, for maximum flexibility in reagent formulation.

Simplify Analysis with Protein Thermal Shift™ Software: Our complete Protein Thermal Shift™ solution includes analysis software which is compatible with run files generated from five of our real-time PCR systems: StepOne™ Real-Time PCR System, StepOnePlus™ Real-Time PCR system, 7500 Fast Real-Time PCR System, and ViiA™7 Real-Time PCR System.

  • Screen samples for relative protein thermal stability
  • Screen molecules (e.g. small molecule drug candidates, fragment-based libraries, and antibodies) for ligand binding to proteins in a high-throughput manner
  • Screen samples for stability changes after protein point mutations or modifications
  • Systematically identify suitable/optimal buffer conditions for the measurement of protein-ligand interactions, production of stable protein preparations, protein crystallization, and storage of proteins
  • Quickly QC protein preps to ensure protein integrity and/or purity
TRUSTED_SUSTAINABILITY

Spécification

Content And Storage Store dye and buffer at 18° to 25°C
Store control ligand and control protein at -15° to -25°C.
Detection Method Primer-probe
PCR Method qPCR
Technique Fluorescence Intensity
For Use With (Equipment) 7500 Fast System, 7500 System, StepOne™, StepOnePlus™, ViiA™ 7 System
Kit Type Starter Kit
Product Line Protein Thermal Shift
Quantity 1,000 reactions
Target Specificity Not Target-Specific
For Use With (Application) Protein Thermal Shift
No. of Reactions 1,000 reactions
Sample Type Protein
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For Research Use Only. Not for use in diagnostic procedures.

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