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Descrizione
Applied Biosystems™ Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest.
Save Time and Money with Sample Screening: This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.
Two Kits to Choose From: The Protein Thermal Shift™ Dye Kit contains the dye and buffer for performing protein thermal shift experiments, enough for up to 1000 reactions. The Protein Thermal Shift™ Starter Kit contains the Dye Kit plus a control protein and control ligand for test experiments and troubleshooting. The Protein Thermal Shift™ Starter Kit components are packaged in two boxes, one to be stored at -20°C and the other at 4°C, for maximum flexibility in reagent formulation.
Simplify Analysis with Protein Thermal Shift™ Software: Our complete Protein Thermal Shift™ solution includes analysis software which is compatible with run files generated from five of our real-time PCR systems: StepOne™ Real-Time PCR System, StepOnePlus™ Real-Time PCR system, 7500 Fast Real-Time PCR System, and ViiA™7 Real-Time PCR System.
- Screen samples for relative protein thermal stability
- Screen molecules (e.g. small molecule drug candidates, fragment-based libraries, and antibodies) for ligand binding to proteins in a high-throughput manner
- Screen samples for stability changes after protein point mutations or modifications
- Systematically identify suitable/optimal buffer conditions for the measurement of protein-ligand interactions, production of stable protein preparations, protein crystallization, and storage of proteins
- Quickly QC protein preps to ensure protein integrity and/or purity
Specifica
Specifica
| Content And Storage | Store dye and buffer at 18° to 25°C Store control ligand and control protein at -15° to -25°C. |
| Detection Method | Primer-probe |
| PCR Method | qPCR |
| Technique | Fluorescence Intensity |
| For Use With (Equipment) | 7500 Fast System, 7500 System, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Kit Type | Starter Kit |
| Product Line | Protein Thermal Shift |
| Quantity | 1,000 reactions |
| Target Specificity | Not Target-Specific |
| For Use With (Application) | Protein Thermal Shift |
| Vedi altri risultati |
For Research Use Only. Not for use in diagnostic procedures.
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