Learn More
Invitrogen™ TRIzol™ Max™ Bacterial RNA Isolation Kit
Description
The TRIzol™ Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol™ Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.
Key Features of the TRIzol™ Max™ Bacterial RNA Isolation Kit:
• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps
Two Products in One Kit
The TRIzol™ Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol™ Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol™ Reagent generates a high-quality, minimally degraded RNA product.
Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol™ Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Specifications
Specifications
| Content And Storage | • 20 mL Max Bacterial Enhancement Reagent; room temperature • 100 mL TRIzol Reagent; room temperature |
| Isolation Technology | Organic Extraction |
| Purification Time | 1 hr. |
| Elution Volume | 50 μL |
| Sample Type | Bacteria (Gram +/-) |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling |
| Quantity | 100 Preps |
| Shipping Condition | Room Temperature |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.