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Invitrogen™ RiboPure™ RNA Purification Kit
Description
The Ambion™ RiboPure™ Kit is designed for rapid purification of high-quality RNA from tissue samples or cultured cells. The kit combines TRI Reagent™ with glass fiber filter purification to yield exceptionally pure RNA, free of residual proteins and lipids. The kit includes sufficient reagents for 50 purifications from 1–100 mg of tissue or 0.1–20 x 106 cultured cells each.
• Yields the highest purity RNA attainable
• Ideal for the most stringent downstream applications
• Handles problematic samples with ease
• Fast (less than 30 minutes) and easy procedure
RNA isolated using the RiboPure™ Kit is suitable for use in all common downstream applications, including cDNA synthesis, real-time and end-point RT-PCR, microarray analysis, northern blots, and RNase protection assays. The RiboPure™ protocol is especially suited for processing samples that are difficult to disrupt, or samples that have large amounts of ribonuclease or other contaminants. Samples are first homogenized in TRI Reagent™, a monophasic solution containing phenol and guanidine thiocyanate, to rapidly lyse cells and inactivate nucleases. Addition of bromochloropropane (BCP) results in separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, while DNA and protein remain in the interphase and organic phase. The aqueous phase is removed and passed through a glass fiber filter that binds the RNA. The filter is washed, and the RNA is eluted. The procedure is compatible with tissues that have been stored in Ambion's RNAlater™. Total RNA yield is typically 100–500 μg per 100 mg of tissue, depending on the type of tissue.
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | • 50 mL TRI Reagent; store at 4°C • 50 Filter Cartridges; room temperature • 100 Collection Tubes (2 mL); room temperature • 60 mL Wash Solution (concentrated); room temperature • 11 mL Elution Buffer; room temperature |
| Isolation Technology | Spin Column (Glass Fiber Filter) |
| Purification Time | 30 min. (After sample homogenization) |
| Elution Volume | 100 μL |
| Sample Type | Cells, Tissue |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Product Type | Kit |
| Quantity | 50 Preps |
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Safety and Handling
- TRI Reagent
- Danger
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 1
- Skin corrosion/irritation Category 1 B
- Specific target organ toxicity after repeated exposure Category 2
- H302-Harmful if swallowed.
- H312-Harmful in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H318-Causes serious eye damage.
- H332-Harmful if inhaled.
- H373-May cause damage to organs.
- P202-Do not handle until all safety precautions have been read and understood.
- P233-Keep container tightly closed.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P271-Use only outdoors or in a well-ventilated area.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P403+P233-Store in a well-ventilated place. Keep container tightly closed.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Phenol, Thiocyanic acid, compound with guanidine
For Research Use Only. Not for use in diagnostic procedures.
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