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Invitrogen™ QuantumRNA™ β-Actin Internal Standards

Product Code. 10744227
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10744227 100 reactions
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Product Code. 10744227 Supplier Invitrogen™ Supplier No. AM1720

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Description

Used for quantitative end-point RT-PCR

The Invitrogen™ QuantumRNA™ β-actin Internal Standards with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal β-actin primers that cannot be extended. By adjusting the ratio of β-actin Competimer™s to normal β-actin mRNA primers, the signal for β-actin mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. β-actin mRNA is an abundantly expressed &ldquohousekeeping” gene; therefore, it is difficult to multiplex primers for β-actin mRNA in the same PCR reaction with primers for rare messages. Typically the β-actin mRNA signal will overwhelm the relative RT-PCR reaction, and the rare gene-specific product goes undetected. β-actin Standards Product Size: 294 bp
TRUSTED_SUSTAINABILITY

Specifications

Product Line Ambion, QuantumRNA
Quantity 100 Reactions
Packaging Tube(s)
Includes β-Actin Primer Pair and β-Actin Competimer™s are stored at –20°C. Control Template RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature.
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