Invitrogen™ Promyelocytic Leukemia (HL-60) Total RNA

The highest-quality RNA available from Invitrogen, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100μg is provided at a concentration of 1mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice™ RNAs. The samples are processed using Invitrogen RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any downstream application, including RT-PCR. Because it is impossible to remove every DNA molecule from an RNA sample, each preparation is tested to ensure that any residual DNA contamination is insignificant. The integrity of the RNA is verified by capillary electrophoresis using the Agilent™ 2100 bioanalyzer.