Acetylcholinesterase Monoclonal Antibody (ZR3), Invitrogen™

Description

MA3-041 contains 100 μL of in vitro produced, protein A purified IgG in PBS containing 1 mg/mL BSA and 0.05% sodium azide. MA3-041 detects acetylcholinesterase (AChE) from feline, human, rat, rabbit and guinea pig tissues. This antibody does not cross react with mouse, chicken, bovine, frog, or electric eel AChE. MA3-041 has been successfully used in immunohistochemistry and immunoprecipitation procedures. This antibody cannot be used in Western blot to detect AChE. Immunohistochemical staining of Ach in rat brain with MA3-041 results in staining of nerve fibers and terminals. MA3-041 reacts with both the soluble and membrane-associated AChE. The MA3-041 antigen is purified rat brain acetylcholinesterase.

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly