missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Real Time PCR Products
  6. Real Time PCR Reagents and Kits
  7. Thermo Scientific™ ABsolute™ Probe-Based Detection qPCR Master Mixes

Thermo Scientific™ ABsolute™ Probe-Based Detection qPCR Master Mixes

Product Code. p-4526932 Shop All Thermo Scientific Products
Click to view available options
Description:
ABsolute QPCR Capillary Mix, 200 rxns
Absolute QPCR ROX (500nM) Mix, QPCR ROX mix; 1.25mL vial; 100 rxns
Absolute QPCR ROX (500nM) Mix, QPCR ROX mix; 5mL Vial; 2000 rxns
Quantity:
10 x 5mL vials; 2000 x 50µL rxns
2 x 1.25mL vials; 100 x 50µL rxns
250 x 20µL rxn
Unit Size:
200 reactions
250 reactions
4000 reactions
This item is not returnable. View return policy

Product Code. 11837682

Brand: Thermo Scientific™ AB1138/A

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

Thermo Scientific™ ABsolute qPCR Master Mixes offer sensitivity and reproducibility for real-time PCR applications.

Enjoy sensitivity and reproducibility in real-time PCR applications with Thermo Scientific™ ABsolute™ Probe-Based Detection qPCR Master Mixes.

ABsolute qPCR Master Mixes are designed to achieve the most consistent and reproducible data across all QPCR platforms. Proprietary additives give enhanced and consistent end point readings as well as low Ct values. All mixes contain Thermo-Start Taq DNA Polymerase, for increased specificity, and dTTP for increased reaction efficiency and sensitivity (low Ct).

  • Maximum specificity—Chemically-modified Thermo-Start™ Taq remains completely inactive until heated to 95°C for 15 minutes, thereby preventing nonspecific amplification

  • Improved efficiency—The use of dTTP in place of dUTP increases reaction efficiency by approximately 5% and therefore reduces reaction time

  • Increased sensitivity—The combination of Thermo-Start Taq and dTTP increases sensitivity and allows for detection of low copy number templates

  • Instrument specific—Individually optimized qPCR mixes designed for all major qPCR / Real-Time PCR instruments

  • Reproducible results—All mixes undergo extensive quality control testing to ensure batch-to-batch consistency

  • Simple protocol—Master mix format reduces protocol steps and minimizes risk of pipetting errors and sample contamination

Includes:

ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated)

Recommended for:

Ideal for qPCR analysis in molecular biology applications, including gene expression and SNP typing

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated). Store at -20°C. Avoid freeze/thaw cycles
Description Absolute QPCR ROX (500nM) Mix, QPCR ROX mix; 1.25mL vial; 100 rxns
Quantity 2 x 1.25mL vials; 100 x 50µL rxns
For Use With (Application) Gene Expression, Genotyping, miRNA analysis
Activation Time heated to 95°C for 15 minutes

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.