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R&D Systems™ Recombinant Human Elongin B/Elongin C/VHL Complex

Product Code. 18748616
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Quantity:
50 μg
Unit Size:
50µg
This item is not returnable. View return policy

Product Code. 18748616

Brand: R&D Systems™ E3600050

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This item is not returnable. View return policy

Description

Together, Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites.

Typical protein concentration for use in vitro will depend on experimental conditions.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q15370.1 (ELOB), Q15369.1 (ELOC), P40337.2 (VHL)
For Use With (Application) Bioactivity
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Molecular Weight (g/mol) M.W. (theoretical): 24 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC)
Purification Method >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain
Quantity 50 μg
Source Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ELOB/ELOC/VHL Complex protein Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met231 - Asp443 (VHL)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method
Conjugate Unconjugated
Buffer X mg/mL (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM DTT
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
Protein ELOB/ELOC/VHL Complex
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