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Abnova™ 293T Whole Cell Lysate (Denatured)

Product Code. 16089645
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Quantity:
100 μL
Unit Size:
100µL
This item is not returnable. View return policy

Product Code. 16089645

Brand: Abnova™ L151V1.100uL

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This item is not returnable. View return policy

Beschreibung

Human Cell Line Lysate

Spezifikation

Cell Line 293T
For Use With (Application) Western Blot
Tissue Kidney
Description Whole cell lysate (denatured)
Lysis Buffer 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Preparation Method Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly.
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 100 μL
Storage Buffer In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Host Species Human
Content And Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Sicherheit und Handhabung

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Produktidentifikator
  • 100UL 293T whole cell lysate (denatured)
 Signalwort
  • Warning
 Gefahrenkategorie
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Gefahrenhinweis
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Sicherheitshinweis
  • P102-Keep out of reach of children.
  • P233-Keep container tightly closed.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Ergänzende Informationen
  • MIXTURE LIST-Contains : 2-mercaptoethanol
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