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Thermo Scientific™ Poly(A) Polymerase, Yeast
Catalyses the addition of adenosine residues onto the 3' ends of RNA. It can be used to add poly(A) tails to RNA in the first step of cloning.
Brand: Thermo Scientific™ 74225Z25KU
Additional Details : Weight : 0.01200kg
Description
- The reaction requires Mn2+ or Mg2+, ATP as substrate, and any RNA containing 3' hydroxyl termini as primer.
- Substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single 3'-dA residue to the ends of the RNA, a useful technique for labeling RNA at the 3' end.
- In comparative studies, yeast poly(A) polymerase works more efficiently than E. coli poly(A) polymerase for RNA oligonucleotide-labeling and poly(A) tailing.
- Poly(A) Polymerase is recommended over T4 RNA Ligase for 3'-end labeling of long RNA molecules.
- Ribonuclease free
- 20 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT, 50% glycerol.
- One unit equals 15 pmol/min AMP incorporated into (riboA)15 at 37°C.
- 600 units/μL
- 3' -end labeling of a ribonucleotide with cordycepin-5'-triphosphate.
- Addition of poly(A) tails to RNA.;2.Labeling the 3' ends of RNA.
- E. coli strain containing an overproducing clone of Yeast Poly(A) Polymerase.<br
Specifications
| Shipped on dry ice. Store at -20°C. | |
| Poly(A) RNA Polymerase |
For Research Use Only. Not for use in diagnostic procedures.