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FAQ
Fetal bovine serum (FBS) is made by collecting blood from the fetuses of pregnant cows during slaughter. The blood is then allowed to clot, and the serum is separated from the clot by centrifugation. The serum is then filtered to remove any remaining cells and debris, resulting in a sterile product used in cell culture and research.
Fetal bovine serum (FBS) is primarily used as a supplement in cell culture media to provide essential nutrients, growth factors, and hormones necessary for the growth and maintenance of eukaryotic cells in vitro. It supports the proliferation of various cell types including mammalian, insect, and plant cells. FBS is widely used in research, biotechnology, and pharmaceutical production.
Yes, some common alternatives include:
- Human serum: Derived from human blood, used in human cell cultures
- Animal-free serum replacements: Synthetic or recombinant products designed to provide similar growth factors and nutrients as FBS
- Serum-free media: Specialized culture media formulated to support cell growth without the need for serum
- Conditioned media: Media that has been conditioned by cells to contain necessary growth factors
- Platelet lysate: Derived from human platelets, used particularly in stem cell cultures
These alternatives can be used depending on the specific needs of the cell culture and the goal of the research.