PCR and qPCR

Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR

Provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR) 250 RXN SSIII 1ST STRAND QPCR SUPERMIX 250 REACTIOns Store at -20°C

Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1mL

Reduces PCR reaction time from 2 hours to as little as 25 minutes X200 MICROAMP, FAST OPTICAL 96-WELL REACTIONplate with barcode, 0.1mL

Applied Biosystems™ MicroAmp™ Optical 8-Cap Strips

Tight seal minimizes evaporation and contamination X300 MICROAMP OPTICAL 8-CAP STRIP STORE AT ROOMtemperature

Invitrogen™ MultiScribe™ Reverse Transcriptase

A recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase that has been optimized for TaqMan-based assays 100 UL MULTISCRIBE REVERSE TRANSCRIPTASE 100µLStore at -20 C

Invitrogen™ Platinum™ Taq DNA Polymerase

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase 120 RXN TAQ DNA POLYMERASE PLATINUM(R)ns Store at -20°C

Invitrogen™ AccuPrime™ Pfx SuperMix

High-fidelity, high-specificity amplification of DNA fragments 200 RXN PFX SUPERMIX 200 REACTIONS STORE AT -20C

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. 100 RXN QRT-PCR SYSTEM ONE-STEP SUPERSCRIPT(TM)III Platinum(r) 100 reactions Store at -20C

Applied Biosystems™ MicroAmp™ Fast 8-Tube Strip, 0.1mL

Maximized thermal conductivity reduces PCR amplification times X125 MICROAMP FAST 8-TUBE STRIP, 0.1ML STORE ATroom temperature

Applied Biosystems™ MicroAmp™ Optical Tube without Cap, 0.2mL

Designed to maximize sample recovery 200 UL MICROAMP OPTICAL TUBE WITHOUT CAP 0.2MLStore at room temperature

Applied Biosystems™ TaqMan™ RNase P Instrument Verification Plates

Preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene. FG,48 WELL LHS RNASEP PLATE

Thermo Scientific™ Maxima™ H Minus First Strand cDNA Synthesis Kits

A complete system for highly efficient synthesis of first strand cDNA. 100RXN Maxima H Minus Kit w/ dsDNase

Eppendorf™ twin.tec™ 96 Well LoBind PCR Plates, Semi-skirted

Single use polycarbonate/polypropylene plates allowing trained specialists to perform PCR and cycle sequencing experiments. Eppendorf™ twin.tec™ 96 Well LoBind PCR Plates are used for PCR in applications including training, routine, and research laboratories in the bioscientific, industrial, and chemical fields X25 TWINTEC PCR PLATE 96 LOBIND SEMISKIRTED, CLEAR

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 100 MLAmbion® Nuclease-free water has not been treated

Applied Biosystems™ MicroAmp™ Optical 8-Tube Strip, 0.2mL

Maximized thermal conductivity X1000 MICROAMP OPTICAL 8-TUBE STRIP, 0.2ML 1000TUBes Store at room temperature

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 8.0 500ML STORE AT ROOM TEMPERature

Thermo Scientific™ T4 DNA Polymerase

Ensure accuracy in DNA synthesis with template-dependent T4 DNA Polymerase that catalyzes the 5'-3' synthesis from primed single-stranded DNA. T4 DNA POL 5U/UL 500U

Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. PHUSION HF MASTER MIX 500 RXNS

Thermo Scientific™ DreamTaq DNA Polymerase

Get higher sensitivity, longer PCR products and higher yields in all standard PCR applications with this enhanced Taq DNA polymerase. X20 Routine PCR, PCR master mixes, DreamTaq(TM)

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA KIT, HIGH CAPACITY CDNA RT1000 reactions

Thermo Scientific™ Phire Buffers

Choose from three formats of these buffers to optimize PCR using Thermo Scientific™ Phire™ Hot Start II DNA Polymerase. DETERGENT-FREE PHIRE BUF 6ML

Applied Biosystems™ MeltDoctor™ HRM Master Mix

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. 50ML MELTDOCTOR HRM

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR

Optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA 50 RXN FIRST STRAND SYSTEM KIT FOR RT-PCR SUPERSCRipt(tm) Store at -20C Ship on Ice Gel Packs

Applied Biosystems™ SimpliAmp™ Thermal Cycler

Small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR SIMPLIAMP THERMAL CYCLER: 3 INDEPENDENT TEMP ZONES

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Save time in PCR applications with Master Mix and get higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. X4 Routine PCR, PCR master mixes, DreamTaq(TM)

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 200 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 200reactions Store at -20C

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA TF,HIGH CAP CDNA REV TRANS KIT

Applied Biosystems™ AmpErase™ Uracil N-Glycosylase (UNG)

Enzyme removes any uracil incorporated into single- or double-stranded DNA 100 UNIT RTTH DNA POLYMERASE, ENZYME + BUFFERpack 100units Store at -20 C

Thermo Scientific™ Phire™ Hot Start II DNA Polymerase

Optimize routine and high throughput PCR applications requiring speed and specifity with this novel hot start DNA polymerase. PHIRE HOT START II 200U

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