Substrates for ELISA Detection

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ OPD (o-phenylenediamine dihydrochloride)

Thermo Scientific™ OPD (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Thermo Scientific™ QuantaBlu™ NS/K Fluorogenic Substrate Kit

Thermo Scientific™ QuantaBlu™ NS/K Fluorogenic Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit

Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit

Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).

BD TMB Substrate Reagent Set

BD TMB Substrate Reagent Set

For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.

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