Antibiotics, Buffers and Supplements

Gibco™ Penicillin-Streptomycin (10,000 U/mL)

For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria PENICILLIN STREPTOMYCIN SOL

Gibco™ TrypLE Express Enzyme (1X), No Phenol Red

Unlike porcine trypsin, free of animal-derived components X20 Tryple express kit Store at 2-8C

Gibco™ N-2 Supplement (100X)

N-2 Supplement (100X) 50ML N2 SUPPLEMENT

Gibco™ Trypsin-EDTA (0.05%), Phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN .05% EDTA

Water, for HPLC-MS, Fisher Chemical

Packaging: Amber glass bottle 1LT Water, for HPLC-MS

Gibco™ TrypLE Express Enzyme (1X), Phenol Red

Animal origin-free, recombinant enzyme X20 Tryple express kit with phenol red store at

Gibco™ Penicillin-Streptomycin (5,000 U/mL)

For prevention from bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. PENICILLIN STREPTOMYCIN SOL

Gibco™ Antibiotic-Antimycotic (100X)

Anti-mycotic, antibiotic for prevention of cell culture contamination ANTIBIOTIC ANTIMYCOTIC

Gibco™ GlutaMAX™ Supplement

An alternative to L-glutamine, with increased stability that improves cell health 100ML GlutaMAX Supplement

Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) (50mg/mL)

Produced by bacterium Micromonospora rhodorangea and acts by binding ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells GENETICIN

Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media 20ML HEPES

Gibco™ Trypsin (2.5%), No Phenol Red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN, 2.5%, 10X

Gibco™ Trypsin-EDTA (0.25%), Phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas TRYPSIN 0.25% EDTA

Gibco™ Trypsin-EDTA (0.5%), No Phenol Red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas 100ML 0.5% Trypsin-EDTA (10x), no phenol redin plastic container, E-Z hold Store at -20C

Corning™ cellgro™ Trypan Blue

Can be used for variety cell counting methods requiring information on cell count and viability. 100 mL Trypan Blue Solution in PBS 0.4% (w/v)

Gibco™ B-27™ Supplement (50X), minus vitamin A

B-27 Supplement (50X), minus vitamin A 100ML B-27 SUPPLEMENT W/O VIT A (50X

Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability 100 ML MEM NON-ESSENTIAL AMINO ACIDS SOLUTION10mM (100X), liquid 100mL RRR - pack at ambient

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression. 5ML Matrigel Growth Factor Reduced (GFR) Basement

Gibco™ Hygromycin B (50mg/mL)

Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus 20 ML HYGROMYCIN B 20ML STORE AT 2-8C

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells INSULIN-TRANS-SEL-G, 100X

Gibco™ Blasticidin S HCl, powder

Bacterial selective antibiotic in concentration range of 50G–100μg/mL 50MG BLASTICIDIN

Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent

StemPro Accutase Cell Dissociation Reagent STEMPRO ACCUTASEStemPro® Accutase® Cell Dissociation Reagent is

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on BD Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses 100ML Cell Recovery Solution

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test TRYPAN BLUE STAIN

Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells 10 ML INSULIN-TRANS-SEL-X (100X), LIQUID STORE AT2-8C

Gibco™ Sodium Pyruvate (100mM)

Growth supplement 100ML Sodium Pyruvate

Corning™ cellgro™ Hygromycin B Solution

Blocks polypeptide synthesis and inhibits elongation. 20 mL Hygromycin B solution

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells. 5ML MatrigelhESC-Qualified Matrix, LDEV-Free

Gibco™ HAT Supplement (50X)

HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells H.A.T. SUPPLEMENT (50X)

Gibco™ KnockOut™ Serum Replacement

KnockOut™ Serum Replacement KNOCKOUT(TM) SR

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