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Invitrogen™ 4X Bolt™ LDS Sample Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
| Buffer | Sample Loading Buffers |
|---|---|
| Content And Storage | Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red. Store at 4°C to 25°C. |
| Gel Type | Bis-Tris Plus |
| Product Type | LDS Sample Buffer |
| For Use With (Equipment) | Bolt Mini Gel Tank |
| Concentration | 4X |
| Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
| Product Line | Bolt™ |
Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels
Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Thermo Scientific™ Pierce™ Protein-Free Blocking Buffer
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.
| Physical Form | Liquid |
|---|---|
| Recommended Storage | Upon receipt store product at room temperature. After opening, store product at 4°C. |
| Concentration | 1X |
| For Use With (Application) | ELISA |
Invitrogen™ TE, pH 8.0, RNase-free
Invitrogen molecular biology grade, TE, pH 8.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use.
| Packaging | Bottle |
|---|---|
| Physical Form | Liquid |
| pH | 8.0 |
| Recommended Storage | Store at room temperature. |
| Product Line | Ambion |
Invitrogen™ NativePAGE™ Sample Prep Kit
Includes sample preparation reagents for native gel electrophoresis
Invitrogen™ NativePAGE™ Sample Buffer (4X)
A solution used to solubilize native protein samples prior to use with the NativePAGE Gel System
Invitrogen™ Tris (1 M), pH 7.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
| Formulation | 1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-,Hydrochloride |
|---|---|
| Physical Form | Liquid |
| pH | 7 |
| Grade | Molecular Biology |
| Recommended Storage | Store at room temperature. |
| Concentration | 1 M |
| Product Line | Ambion |
Invitrogen™ TAE (10X), RNase-free
Molecular biology grade, Invitrogen 10X TAE is supplied in a 1 L bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Invitrogen™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.
| Content And Storage | Sample Reducing Agent (10X) contains 500 mM dithiothreitol (DTT). Store at 2°C to 8°C. |
|---|---|
| Form | Liquid |
| Product Type | Sample Reducing Agent |
| For Use With (Equipment) | Bolt Mini Gel Tank |
| Formulation | 500 mM dithiothreitol (DTT) |
| Reagent Type | Reducing Agent |
| Concentration | 10X, 500 mM |
| Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
| Product Line | Bolt™ |
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
| Content And Storage | Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restore™ |
Thermo Scientific™ Pierce™ Fast Blocking Buffer
Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with this ready-to-use blocker solution.
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | Blocking Buffer |
| Recommended Storage | Upon receipt store at 4°C. |
| Concentration | 1X |
| For Use With (Application) | Western Blot |
Invitrogen™ 20X Bolt™ MOPS SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
| For Use With (Equipment) | Bolt |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Running Buffer |
| Concentration | 20X |
| Product Line | Bolt |
Thermo Scientific™ Agarase (0.5 U/μL)
Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.