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1
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Thermo Scientific™ FastDigest AjuI

Cut at ^(7/12)GAA(N)7TTGG(11/6)^ sites with Thermo Scientific™ FastDigest™ AjuI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

EDGE
2
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Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Not I
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 80°C
Product Line FastDigest™
3
Promotions Available

Thermo Scientific™ FastDigest EheI

Cut at GGC^GCC sites with Thermo Scientific™ FastDigest™ EheI, which performs best using universal FastDigest Buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).

Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme EheI
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang Blunt
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
4

Invitrogen™ Anza™ T4 DNA Ligase Master Mix

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

Shipping Condition Approved for shipment on Wet or Dry Ice
Product Type T4 DNA Ligase Master Mix
Enzyme T4 DNA Ligase
Compatible Buffer Elution Buffer, 1X Anza Buffer
Concentration 4X
Product Line Anza™
5

MP Biomedicals™ Zymolyase™

For lysing the cell walls of viable yeast cells and preparing yeast spheroblasts

Content And Storage 2°C to 8°C
Form Lyophilized Powder
pH 5 to 10
For Use With (Application) Used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of Yeast cells
Source Arthrobacter luteus