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ABI Applied Biosystems (360)
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PCR (1)
Scarless Cloning (62)
Signal Transduction (1)
Traditional Cloning (613)

Optimal Reaction Temperature

+4°C to +30°C (2)
30°C (9)
37°C (594)
50°C (2)
55°C (21)
65°C (25)

Sensitive to Heat Inactivation

No (92)
Yes (558)

Methylation Sensitivity

CpG Methylation-Sensitive (312)
Dam Methylation-Sensitive (48)
Dcm Methylation-Sensitive (43)
Methylation Sensitivities Varies by Enzyme (2)
Not CpG Methylation-Sensitive (373)
Not Dam Methylation-Sensitive (594)
Not Dcm Methylation-Sensitive (563)

GC-Rich PCR Performance

High (366)
Low (113)
Medium (33)

1 – 15 of 1,889 results

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

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Specifications

Real Time PCR Master Mixes

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

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Specifications

Real Time PCR Master Mixes

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq DNA Polymerase
Format	Tube
Product Type	Fast Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Product Line	AmpErase™, TaqMan™

Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

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Pricing and Availability
Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Invitrogen™ Fixative-Free Lysing Solution, High-Yield Lyse

Fixative-Free Lysing Solution, High-Yield Lyse

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DNA Extraction and Purification

Applied Biosystems™ GeneAmp™ dNTP Blend (2.5 mM ea)

GeneAmp dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial consists of 1 mL of 2.5 mM each of dATP, dCTP, dGTP, and dTTP, yielding a 10 mM blend of dNTPs.

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Specifications

dNTPs

Shipping Condition	Dry Ice
Form	Liquid
Concentration	10 mM
For Use With (Application)	Standard PCR
Product Line	GeneAmp™

Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits

Highly selective for dsDNA over RNA, the Qubit 1X dsDNA HS and BR Assay Kits offer easy-to-use formulations for the accurate quantitation of dsDNA in 100 and 500 assay formats. Lambda Standards provide sufficient material for up to 500 samples.

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Nucleic Acid Labeling and Detection

Green Features	Fewer resources used, Less waste
Product Line	Qubit

Invitrogen™ Qubit™ dsDNA Quantification Assay Kits

For highly selective detection of dsDNA over RNA, even in the presence of contaminants. The Qubit dsDNA Assay Kits are accurate from 10 pg/uL to 100 ng/uL, and 100 pg/uL to 1,000 ng/uL, respectively.

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Specifications

Nucleic Acid Labeling and Detection

Shipping Condition	Room Temperature
Excitation/Emission	510/527
For Use With (Equipment)	Qubit Fluorometer
Product Line	Qubit
Detection Method	Fluorescence

Invitrogen™ Lipofectamine™ 2000 Transfection Reagent

Versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines

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Transfection Reagents

Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor

Protects mRNA and improves total cDNA yields

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RNA Preparation and Purification

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

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Specifications

cDNA Synthesis Kits and Master Mixes

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	MultiScribe
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	120 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Concentration	50 U/μL
Technique	Reverse Transcription
Starting Material	RNA

Invitrogen™ TRIzol™ Reagent

TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.

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Specifications

RNA Isolation and Purification Products

Shipping Condition	Room Temperature
Final Product Type	Total RNA, DNA, Protein
Sample Type	Bacteria,Cells,Plants,Tissue,Yeast
Isolation Technology	Organic Extraction
Starting Material Amount	Bacteria: ≤10⁷ cells Cells: ≤10⁷ Plant: ≤10⁷ cells, 100 mg Tissue: ≤100 mg Yeast: ≤10⁷ cells
Purification Time	1 hr.
Yield	DNA: ≤7 μg RNA: ≤73 μg Protein: varies
Elution Volume	20 to 600 μL
For Use With (Application)	RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
High-throughput Compatibility	Not High-throughput Compatible (Manual)

Thermo Scientific™ GeneJET Plasmid Miniprep Kit

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.

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Pricing and Availability
Specifications

DNA Extraction and Purification

Target	Plasmid DNA
Format	Spin Column
Product Type	Plasmid MiniPrep Kit
Sample Type	Bacteria
Isolation Technology	Silica Column
Column Type	Spin Column
Scale	Mini
High-throughput Compatibility	Compatible (Vacuum manifold)
For Use With (Application)	PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
Test Time	<14 min
Product Line	GeneJET
Prep Scale	< 100 μg (Small-Scale) Plasmid DNA

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

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Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Content And Storage	• Phusion DNA Polymerase (2 U/μL) • 5X Phusion HF & GC Buffers • DMSO • 50 mM MgCl₂ solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl₂ in the final 1X concentration. Store at -20°C.
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Product Type	High-Fidelity DNA Polymerase
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2 U/μL
For Use With (Application)	Standard PCR,High-fidelity PCR

Invitrogen™ Gateway™ LR Clonase™ II Enzyme mix

Contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.

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Specifications

Modifying Enzymes

Shipping Condition	Dry Ice
Content And Storage	Gateway™ LR Clonase™ II enzyme mix includes proteinase K solution (2 μg/μL) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored.
Product Type	LR Clonase Enzyme Mix
Enzyme	LR Clonase
Compatible Buffer	Enzyme Buffer
Product Line	Clonase™, Gateway™

Invitrogen™ Lipofectamine™ RNAiMAX Transfection Reagent

Provides highest transfection efficiencies on widest variety of cell types for siRNA-mediated gene knockdown experiments

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Pricing and Availability
Specifications

Transfection Reagents

Shipping Condition	Approved for shipment at Room Temperature or on Wet Ice
Format	6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Product Type	Transfection Reagent
Transfection Technique	Lipid-based Transfection
Serum Compatible	Yes
Green Features	Sustainable packaging
High-throughput Compatibility	High-throughput Compatible
For Use With (Application)	Transfection
Product Line	Lipofectamine™
Cell Type	Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells

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Invitrogen™ Qubit™ dsDNA Quantification Assay Kits Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ Lipofectamine™ 2000 Transfection Reagent Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ Lipofectamine™ RNAiMAX Transfection Reagent Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ Qubit™ dsDNA Quantification Assay Kits

Invitrogen™ Lipofectamine™ 2000 Transfection Reagent

Invitrogen™ Lipofectamine™ RNAiMAX Transfection Reagent