Miscellaneous Molecular Biology Reagents
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Poloxamer 188
Molecular Formula: C5H10O2 Synonym: Pluronic™ F-68; Polyoxyethylene-polyoxypropylene block copolymer
Invitrogen™ EDTA (0.1 mM), pH 8.0, RNase-free
Invitrogen 0.1 mM EDTA is a commonly recommended storage solution for RNA samples. 0.1 mM EDTA is in nuclease-free, ultrapure water. Supplied in one bottle of 50 mL.
Invitrogen™ UltraPure™ BSA (50 mg/mL)
Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.
Invitrogen™ NaCl (5 M), RNase-free
Invitrogen Molecular biology grade, 5 M NaCl solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Thermo Scientific™ DTT (dithiothreitol)
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Invitrogen™ SDS, 20% Solution, RNase-free
Invitrogen molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL.
Invitrogen™ PureLink™ RNase A (20 mg/mL)
PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA.
Thermo Scientific™ EDTA
Chelate alkaline earth metals. Thermo Scientific™ Pierce EDTA is highly purified for use in a variety of laboratory methods.
Invitrogen™ UltraPure™ Guanidine Isothiocyanate Solution
UltraPure Guanidine Isothiocyanate Solution consists of 4M guanidine isothiocyanate, 50 mM Tris-HCl (pH 7.5), 25 mM EDTA.
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.
Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
Thermo Scientific™ X-Gal
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 μm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR.
Thermo Scientific™ MassRuler DNA Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X MassRuler DNA Loading Dye.