Cell Based Assays
Invitrogen™ Calcein AM, Cell-permeant Green and Blue Dyes
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
Invitrogen™ CM-H2DCFDA (General Oxidative Stress Indicator)
General oxidative stress indicator
Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570)
Sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red™ filters
Invitrogen™ CyQUANT™ Cell Proliferation Assays
CyQUANT cell proliferation assays are fast and highly sensitive fluorescence methods for counting cells to assess cell growth and cytotoxicity in microplate format. These assays use fluorescent nucleic-acid-binding dyes to measure cell number based on DNA content.
Invitrogen™ JC-1 Dye (Mitochondrial Membrane Potential Probe)
Sensitive marker for mitochondrial membrane potential
Invitrogen™ CellMask™ Plasma Membrane Stains
Invitrogen CellMask™ plasma membrane stains enable fast and uniform labeling of the plasma membrane in almost all cell types. These stains work rapidly and can be detected using standard microscope filter sets with any imaging instrument.
Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Useful with a variety of fluorescence-detection methodologies
Molecular Probes™ Propidium Iodide - 1.0 mg/mL Solution in Water
Propidium Iodide - 1.0 mg/mL Solution in Water
Invitrogen™ Tetramethylrhodamine, Methyl Ester, Perchlorate (TMRM)
For use with fluorescence microscopy
Invitrogen™ CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry
CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.
Invitrogen™ MitoTracker™ Dyes for Mitochondria Labeling
These mitochondria dyes are specially packaged in vials of 50 μg each to create freshly reconstituted dyes without the impact of freeze-thaw cycles to aid in a simplified sample prep for cell analysis workflow.
Invitrogen™ SYTO™ Green Fluorescent Nucleic Acid Stains
SYTO Green Fluorescent Nucleic Acid Stains exhibit bright, green fluorescence upon binding to nucleic acids. These dyes are cell-permeant and can be used to stain RNA and DNA in live cells, dead cells, and bacteria.
Molecular Probes™ Fluo-4, AM, cell permeant
Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.
Molecular Probes™ MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
Invitrogen™ LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation
Determines the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation.