PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.

Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.

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Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

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Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, without competent cells

Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products

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Specifications

Shipping Condition	Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoter	T3, T7
Content And Storage	Box 1: Store at -5 to -30°C. • pCR XL-2-TOPO vector • Salt Solution • T3 Primer • T7 Primer • XL Control PCR Template • XL Control PCR Primers • Platinum SuperFi Green PCR Master Mix • SuperFi GC Enhancer • Nuclease-free water Box 2: Store at room temperature. • Gel Solubilization Buffer (L3) • Binding Buffer (B2) • Wash Buffer (W1) • Elution Buffer (E1) • PureLink Clean-up Spin Columns (in Wash Tubes) • PureLink Recovery/Elution Tubes
Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	Cloning Kit
Cloning Method	Zero Blunt TOPO
Bacterial or Yeast Strain	Not Included
For Use With (Application)	PCR Cloning
Vector	pCR-XL-2-TOPO
Product Line	TOPO

Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1^R Chemically Competent E. coli Cells

Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoter	T3, T7
Content And Storage	Box 1: Store at -5 to -30°C. • pCR XL-2-TOPO vector • Salt Solution • T3 Primer • T7 Primer • XL Control PCR Template • XL Control PCR Primers • Platinum SuperFi Green PCR Master Mix • SuperFi GC Enhancer • Nuclease-free water Box 2: Store at room temperature. • Gel Solubilization Buffer (L3) • Binding Buffer (B2) • Wash Buffer (W1) • Elution Buffer (E1) • PureLink Clean-up Spin Columns (in Wash Tubes) • PureLink Recovery/Elution Tubes Box 3: • One Shot OmniMAX 2 T1R chemically competent E. coli • S.O.C. Medium • pUC 19 Control DNA Store competent cells and pUC19 at -65 to -85°C.
Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	Cloning Kit
Cloning Method	Zero Blunt TOPO
Bacterial or Yeast Strain	OmniMAX2
For Use With (Application)	PCR Cloning
Vector	pCR-XL-2-TOPO
Product Line	TOPO
Cell Type	Chemically Competent E. coli

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R E. coli and PureLink™ Kit

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

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PCR Cloning Kits

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