PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.

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Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Pricing and Availability
Specifications

Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Format	Kit
Sample Type	PCR Products
Cloning Method	Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application)	PCR Cloning
Vector	pJET1.2/blunt
Product Line	CloneJET

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Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Pricing and Availability
Specifications

Promoter	T7
Content And Storage	Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt PCR
Bacterial or Yeast Strain	Not Included
Vector	Blunt DNA Cloning Vectors
Product Line	TOPO, Zero Blunt

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

Pricing and Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	TOP10
Cell Type	Chemically Competent

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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

Pricing and Availability
Specifications

Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Product Line	TOPO

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Pricing and Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial	Kanamycin (KanR), Zeocin™ (ZeoR)
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™

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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10F' E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Pricing and Availability
Specifications

Content And Storage	Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10F´
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot

Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector, without competent cells

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Pricing and Availability
Specifications

Promoter	T7, SP6
Content And Storage	This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TA Cloning
Bacterial or Yeast Strain	Not Included
For Use With (Application)	PCR Cloning
Vector	pCRII
Product Line	TA Cloning

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells

Pricing and Availability
Specifications

Promoter	T7, SP6
Content And Storage	Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Cloning Method	TOPO™-TA
Vector	TOPO-TA Vectors
Product Line	TOPO

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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR™4-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

Provides a 5 minute, one-step cloning strategy. Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR4-TOPO Vector, One Shot™ TOP10 Chemically Competent E. coli and PureLink Plasmid Prep Kit enables direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing.

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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli

Provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli is available in 20- and 40-reaction kit sizes.

Pricing and Availability
Specifications

Promoter	T7
Content And Storage	TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TA Cloning
Bacterial or Yeast Strain	TOP10
For Use With (Application)	PCR Cloning
Product Line	One Shot
Cell Type	Chemically Competent E. coli

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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Pricing and Availability
Specifications

Promoter	T7
Content And Storage	TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TA Cloning
Bacterial or Yeast Strain	Not Included
For Use With (Application)	PCR Cloning
Vector	pCR2.1
Product Line	TA Cloning

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Promoter	T7, T3
Content And Storage	Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Vector	TOPO Cloning Vectors
Product Line	TOPO

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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Pricing and Availability
Specifications

Content And Storage	Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10
Cell Type	Chemically Competent

PCR Cloning Kits

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