Real Time PCR Assays and Arrays

Real Time PCR Assays and Arrays
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Applied Biosystems™ TaqMan™ MicroRNA Assay
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Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.
Shipping Condition | Room Temperature |
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GC-Rich PCR Performance | High |
Content And Storage | • 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size) • 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers). Store at -15 to -25°C. |
Format | Tube |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex |
Reaction Speed | Fast |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
TaqMan® TaqMan™ Gene Expression Assay (FAM)
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Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.
Shipping Condition | Room Temperature |
---|---|
GC-Rich PCR Performance | High |
Content And Storage | 1 tube containing 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers). Store at -15°C to -25°C. |
Fidelity (vs. Taq) | 60 X |
Product Type | Gene Expression Assay |
Reaction Speed | Fast |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
TaqMan® TaqMan™ Gene Expression Assay, VIC
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Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.
GC-Rich PCR Performance | High |
---|---|
Fidelity (vs. Taq) | 60 X |
Format | Tube |
Product Type | Gene Expression Assay |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex |
Reaction Speed | Fast |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Copy Number Reference Assay, human, RNase P
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Human TaqMan™ Copy Number Reference Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome.
Shipping Condition | Ambient Temperature |
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GC-Rich PCR Performance | Low |
Content And Storage | Control Primer-Probe Set(s) |
Purity or Quality Grade | RNase-Free, DNase-Free |
Internal Probe Modification | TAMRA™ Quencher (3'), VIC™ (5') |
Form | Frozen |
Gene Symbol | RPPH1 |
Green Features | Sustainable packaging |
Concentration | 20X |
PCR Method | qPCR |
Detection Method | Primer-probe |
Target | RNase P |
Purification Method | HPLC |
Format | Tube |
Product Type | Configurable Assay |
Reaction Speed | Standard |
5'Primer Modification | None |
Label or Dye | VIC |
3'Primer Modification | None |
Species | Human |
Product Line | TaqMan™ |
Applied Biosystems™ TaqMan™ Copy Number Reference Assay, mouse, Tfrc
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Mouse TaqMan™ Copy Number Reference Assays are run with predesigned, Custom Plus, and Custom TaqMan™ Copy Number Assays in a duplex, real-time PCR reaction to detect and measure copy number variations in the mouse genome.
Format | Tube |
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PCR Method | qPCR |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ MGB Probe
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Custom TaqMan™ Probe
Shipping Condition | Room Temperature |
---|---|
Purification Method | HPLC |
Purity or Quality Grade | HPLC |
Format | Tube |
Internal Probe Modification | None |
Product Type | TaqMan Probe |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex |
Form | Liquid |
5'Primer Modification | FAM, NED, TET, VIC |
Green Features | Sustainable packaging |
3'Primer Modification | MGB-NFQ |
For Use With (Application) | Real Time PCR (qPCR) |
Product Line | TaqMan™ |
Applied Biosystems™ TaqMan™ Gene Expression Assay, VIC primer-limited
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Applied Biosystems TaqMan Gene Expression Assays, VIC primer-limited, are used for quantitative real-time PCR analysis of gene expression.
Shipping Condition | Room Temperature |
---|---|
GC-Rich PCR Performance | High |
Content And Storage | 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers). Store at -15 to -25°C. |
Fidelity (vs. Taq) | 60 X |
Format | Tube |
Product Type | Gene Expression Assay |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex |
Reaction Speed | Fast |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)
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Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)
Applied Biosystems™ Human GUSB (Beta Glucuronidase) Endogenous Control (FAM™/MGB probe, non-primer limited)
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Human GUSB (Beta Glucuronidase) Endogenous Control (FAM™/MGB probe, non-primer limited)
Shipping Condition | Room Temperature |
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Content And Storage | Control Primer-Probe Set(s) |
Gene Symbol | GUSB |
Green Features | Sustainable packaging |
Amplicon Size | 81 |
Concentration | 20X |
For Use With (Application) | Gene Expression |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Accession Number | NM_000181.1 |
Target | GUSB |
Format | Tube |
Label or Dye | FAM |
Species | Human |
Product Line | FAM™, TaqMan™ |
Applied Biosystems™ Mouse GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)
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Mouse GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)
Applied Biosystems™ Human RPLPO (Large Ribosomal Protein) Endogenous Control (FAM™/MGB probe, non-primer limited)
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Human RPLPO (Large Ribosomal Protein) Endogenous Control (FAM™/MGB probe, non-primer limited)
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Control Primer-Probe Set(s) |
Gene Symbol | RPLPO |
Green Features | Sustainable packaging |
Amplicon Size | 105 |
Concentration | 20X |
For Use With (Application) | Gene Expression |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Accession Number | NM_053275.3 |
Target | RPLPO |
Format | Tube |
Label or Dye | FAM |
Species | Human |
Product Line | FAM™, TaqMan™ |
Applied Biosystems™ Human GUSB (Beta Glucuronidase) Endogenous Control (VIC™/TAMRA™ probe, primer limited)
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Human GUSB (Beta Glucuronidase) Endogenous Control (VIC™/TAMRA™ probe, primer limited)
Applied Biosystems™ Human TBP (TATA-box binding Protein) Endogenous Control (VIC™/MGB probe, primer limited)
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Human TBP (TATA-box binding Protein) Endogenous Control (VIC™/MGB probe, primer limited)
Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)
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Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)
Applied Biosystems™ Human TFRC (CD71) (Transferrin Receptor) Endogenous Control (VIC™/MGB probe, primer limited)
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Human TFRC (CD71) (Transferrin Receptor) Endogenous Control (VIC™/MGB probe, primer limited)