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Optimised and Efficient Protein Sample Preparation
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Optimised and Efficient Protein Sample Preparation

Protein extraction, purification, and cleanup products.

Why choose Thermo Scientific™ Protein Sample Preparation

Protein Extraction Reagents and Kits

We offer a full range of optimised protein extraction products designed to maximize protein yield and activity. The gentle formulations have been validated with multiple sample types to eliminate mechanical disruption and preserve protein activity.

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Protein Cleanup

Our easy-to-handle devices offer high performance for secure sample processing and protein recovery, removing unwanted small molecules and salts, to help maintain protein integrity and purity for subsequent downstream applications.

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Protein Purification

Our high-performance resins and magnetic beads offer a broad range of formats for ion exchange and affinity-based isolation of proteins and antibodies in microgram to kilogram quantities.

Purification Icon

Protein Sample Preparation

Optimised and Efficient Protein Sample Preparation

Extract. Clean up. Purify.

Our convenient devices and high-performance affinity resins and magnetic beads enable maximum yield for the purification, enrichment, and cleanup of proteins and antibodies for downstream applications.

Protein sample preparation is a process which includes protein extraction through cell disruption, protein preservation using protease and phosphatase inhibitors, protein isolation and purification, buffer clean-up through dialysis, desalting and concentration, and quantitation with assay methods that may use fluorescent or colorimetric detection.

Protein preparation is an important step before proteomics workflows involving mass spectrometry or gel electrophoresis. These products are optimized for a wide range of tissue and cell types and are compatible with a broad range of protein purification resins and formats.

Desalt, buffer exchange, remove contaminants, and concentrate proteins with our secure and efficient devices and resins.

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Protein Extraction Reagents and Kits

Gentle formulations designed to maximize protein yield and activity:

  • Optimized—formulations maximize protein yield and preserve protein activity
  • Efficient—minimizing cross-contamination between subcellular fractions
  • Compatible—extracts can be used directly in most downstream applications
  • Gentle—eliminates the need for mechanical cell disruption for most sample types

Slide-A-Lyzer Dialysis Products

Easy-to-handle devices, cassettes, and flasks for secure sample processing:

  • Excellent sample recovery—low-binding plastics and membranes help minimize sample loss compared to filtration and resin systems
  • Convenient—easy-to-grip design helps simplify sample addition and removal with syringe and/or pipette
  • Secure—sealed membranes help prevent leakage that can occur with dialysis tubing and homemade devices
  • Validated—each device is leak-tested during production

Zeba Desalting Products

Convenient spin formats help ensure rapid desalting with high protein recovery:

  • High performance—proprietary resin enables excellent protein recovery and efficient contaminant removal
  • Flexible—available in spin columns, filter spin plates, and cartridges for a range of needs
  • Fast—no fraction screening or waiting for protein to emerge by gravity flow
  • Economical—cost-effective products that offer great performance

Protein Purification Products

High-performance resins and magnetic beads for maximum protein yield:

  • Broad product selection—strong ion exchange and affinity supports for the purification and enrichment of proteins and antibodies; affinity ligands enable 1-step purification of recombinant and biotinylated proteins, while activated supports provide a platform for custom protein immobilization
  • High performance—resins are designed to maximize protein yield and reduce background
  • More formats—magnetic beads, loose resins, FPLC cartridges, and 96-well filter plates enable protein purification from screening and small-scale phases to process-scale purification
  • Economical—pricing that is similar to or better than other leading suppliers

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