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PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
PCR Supplies (1,687)
Thermal Cyclers (267)
Oligonucleotides (238)
Thermal Cycler Accessories (224)

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1 – 15 of 1,471 results
1

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

EDGE
Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
For Use With (Application) RT-PCR
Format Kit
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
2
Promotions Available

Invitrogen™ Platinum™ SuperFi II PCR Master Mixes

Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.

EDGE
Shipping Condition Dry Ice
Polymerase Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >300X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
3
Promotions Available

Applied Biosystems™ QuantStudio™ 3/5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, SYBR™ dyes), 96-well, 0.1 mL

Plate establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, and SYBR™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1mL RT-PCR systems.

EDGE
4
Promotions Available

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

EDGE
GC-Rich PCR Performance High
Format Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Quantity Each
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
5
Promotions Available

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

EDGE
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan
6
Promotions Available

Thermo Scientific™ dNTP Mix (25 mM each)

Thermo Scientific dNTP Mix (25 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 25 mM.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 25 mM
For Use With (Application) Standard PCR,High-fidelity PCR,RT-PCR,Real Time PCR (qPCR)
7

Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.

EDGE
No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell E.coli
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
8
Promotions Available

Invitrogen™ dNTP Mix (10 mM ea)

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.

EDGE
Shipping Condition Approved for shipment on Wet or Dry Ice
Form Liquid
Label or Dye Unlabeled
Concentration 10 mM
For Use With (Application) Standard PCR,RT-PCR,Real Time PCR (qPCR)
9
Promotions Available

Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

EDGE
Shipping Condition Dry Ice
Polymerase Phusion Plus DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >100X
Size (Final Product) 20 kb or less
Reaction Format Ready to use master mix
Reaction Speed 15 to 30 sec./kb
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
10
Promotions Available

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Platinum SuperFi DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 300X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
11
Promotions Available

Thermo Scientific™ dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 10 mM
12
Promotions Available

Thermo Scientific™ Aminoallyl-dUTP Solution (10 mM)

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

EDGE
13
Promotions Available

Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

TaqMan™ RNase P Detection Reagents Kit

EDGE
14

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

EDGE
Shipping Condition Dry Ice
Reverse Transcriptase M-MLV
Form Liquid
InhibitorToleranceSimple High
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 3-plex
Detection Method Primer-probe
Thermostability Not Applicable
Polymerase AmpliTaq Fast DNA Polymerase
Format Tube
Product Type 1-Step Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Hot Start Built-In Hot Start
Product Line TaqMan
15
Promotions Available

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR