MultiShot Competent Cells

Optimized for high-throughput cloning workflows, delivering superior transformation efficiency, scalability, and reproducibility.

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How Can Invitrogen™ MultiShot™ Competent Cells Help You?

Exceptional Transformation Efficiency

High transformation efficiency supports large library construction, complex assemblies, and reliable clone recovery in high-throughput workflows.

Optimized for Automation and Scale

Validated for use in automated liquid handling systems and 96-well plate formats to streamline screening and colony picking.

Consistent, Reproducible Performance

Manufactured under rigorous quality controls to ensure lot-to-lot consistency and reliable cloning results across experiments.

Applicable Solutions

Cloning and Transformation

High-efficiency chemical transformation of plasmid DNA for molecular cloning, gene synthesis, CRISPR plasmid construction, DNA library generation, and synthetic biology workflows. Suitable for routine cloning, plasmid propagation, and downstream protein expression (strain dependent: TOP10, BL21(DE3), Stbl3, DH5α T1R and Mach 1 T1R).

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Cloning and Expression Workflow Compatibility

Compatible with common DNA assembly and cloning methods including Gibson Assembly, Golden Gate Assembly, TOPO cloning, restriction enzyme-based cloning, and ligation workflows. Selected strains support high-copy plasmid propagation (TOP10, DH5α T1R, Mach 1 T1R), stable maintenance of repetitive or lentiviral constructs (Stbl3), and IPTG-inducible recombinant protein expression using T7 promoter systems (BL21(DE3)).

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Frequently Asked Questions

The choice between electroporation and chemical transformation is determined by factors such as the required transformation efficiency, the size and amount of DNA to be introduced, and the equipment available in the laboratory.

Transformation efficiencies ranging from 10⁶ to 10¹⁰ CFU/µg are generally sufficient for most molecular cloning applications.

Competent cell genotypes and selectable markers should be carefully assessed to ensure they align with the intended cloning or expression application.