Phenomenex

Solid Phase Extraction (SPE) is a sample preparation extractive technique used in laboratories to clean-up, solvent switch, and concentrate samples prior to analysis with methods like HPLC or GC. Solid phase extraction is essential to achieve effective separations from complex mixtures by selectively isolating target analytes whilst removing unwanted contaminants, thereby achieving more accurate and precise analytical results.

Supported Liquid Extraction (SLE) is a simple, reliable sample preparation technique that delivers cleaner extracts with minimal hands-on time. By performing liquid–liquid partitioning on an inert solid sorbent rather than in conventional liquid-liquid extraction (LLE), SLE avoids emulsion formation, inconsistent mixing, and manual phase separation. The aqueous sample is immobilized within the sorbent bed, enabling a water-immiscible organic solvent to pass through and extract analytes with high efficiency. The result is a fast, reproducible workflow ideal for subsequent LC and GC analyses.

Phospholipids reduce HPLC/UHPLC column lifetime and sensitivity and are also responsible for ion suppression. Phospholipids are present in many bioanalytical samples including whole blood and plasma. As the number of applications being developed from bioanalytical samples increases, the removal of phospholipids from the sample prior to LC-MS/MS analysis has become an important step for accurate analysis.

Proteins in biological sample matrices can significantly shorten HPLC/UHPLC column lifetimes and can interfere with MS detector sensitivity. Protein precipitation is a simple sample preparation technique that is used to remove proteins from the sample using an organic solvent or a salt.

Oligonucleotide purification is the process of isolating full-length synthetic DNA or RNA sequences from impurities generated during chemical synthesis. These impurities may include truncated sequences, incomplete coupling products, residual protecting groups, salts, and organic reagents. Effective purification ensures recovery of only the target oligonucleotide with the correct sequence and chemical integrity for downstream use in applications such as PCR, gene synthesis, and therapeutic development. As a result, accurate and efficient oligonucleotide purification is a critical step in maintaining sequence  integrity, high purity, and reproducibility across both research and manufacturing workflows.