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Rat spleen tissue lysate, Non-denatured; Abnova

Catalog No. 16099605
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Menge:
200 μg
Packungsgröße:
200 Mikrogramm
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Catalog No. Menge Packungsgröße
16099605 200 μg 200 Mikrogramm
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Catalog No. 16099605 Lieferant Abnova Lieferanten-Nr. L042W2.200ug
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Estimated Shipment: 17-12-2026
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Description

Non denatured tissue lysate of Rat spleen

  • Tissue: Spleen
  • Host: Rat
  • Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

Zur Verwendung mit (Anwendung) Immunoprecipitation, Western Blot
Tissue Spleen
Beschreibung Whole tissue lysate (non-denatured)
Lysepuffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Vorbereitungsmethode Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Qualitätskontrollen 12.5% SDS-PAGE Stained with Coomassie Blue.
Menge 200 μg
Lagerungspuffer In modified RIPA Lysis Buffer.
Wirtsspezies Rat
Konzentration 5 mg/mL
Inhalt und Lagerung Lagerung bei -80 °C. Aliquot, um wiederholtes Einfrieren und Autauen zu vermeiden.
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