Invitrogen™ Mouse TRANCE (RANKL) Recombinant Protein

Description

Receptor Activator of NF-kB Ligand (RANK Ligand) is a cell-bound marker (CD254) related to the TNF family of proteins. RANK Ligand plays a critical role in bone metabolism,particularly osteoclast differentiation. In addition,RANK Ligand is expressed by some T cells is thought to promote dendritic cell maturation. Recombinant mouse RANK Ligand is a non-glycosylated protein,containing 175 amino acids,with a molecular weight of 19.5 kDa. Purity is typically greater than 95% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined in RAW-Blue™ cells and is typically less than 50ng/mL. The specific activity of this protein is 2x10^4 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at-20°C and add 0.1% BSA for additional stability. Amino acid sequence: MPAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID

Receptor Activator of NF-kB Ligand (RANK Ligand) is a cell-bound marker (CD254) related to the TNF family of proteins. RANK Ligand plays a critical role in bone metabolism, particularly osteoclast differentiation. In addition, RANK Ligand is expressed by some T cells is thought to promote dendritic cell maturation. Recombinant mouse IGF-I is a non-glycosylated protein, containing 70 amino acids, with a molecular weight of 7.6 kDa. Purity is typically greater than 95% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent proliferation of FDC-P1 cells and is typically less than 20 ng/mL. The specific activity of this protein is 5x10^4 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA.