missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit

Optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in cloning, PCR, and qPCR.

Brand:  Invitrogen™ 11750350

3441.28 EUR valid until 2021-12-22
Use promo code "16346" to get your promotional price.



Additional Details : Weight : 0.10500kg

 View more versions of this product

Product Code. 16653873

  • 4528.00 € / Each

Estimated Shipment: 22-12-2021
Add to basket

For Research Use Only. All usage must comply with product instructions.


Description

Description

Features of the SuperScript™ IV kit include:

  • Compatible with a wide range of mammalian cell types grown under different treatment conditions
  • Single-tube format minimizes reagent loss, sample loss, and handling time
  • Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
  • High-quality cDNA generated for use in a variety of applications, including cloning, PCR, and qPCR
  • Simple protocol takes around 37 minutes
  • Compatible with TaqMan™ and SYBR Green qPCR master mixes from various manufacturers
  • Optimized performance for 1–10,000 cells per sample; results equivalent to those from purified RNA

The SuperScript™ IV CellsDirect cDNA Synthesis Kit includes SuperScript™ IV Reverse Transcriptase. Features of this enzyme include:

  • Reduced RNase H activity
  • Higher thermal stability
  • High yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts
  • Superior tolerance to various RT inhibitors

SuperScript™ IV reverse transcriptase is a top choice for all RT-PCR and qRT-PCR applications. It is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript™ III reverse transcriptase in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity.

How it works
In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material. Using the SuperScript™ IV CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I treatment is an optional step and can be used to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell.

Specifications

Specifications

RT-PCR (Endpoint), Real-Time PCR
SuperScript™
Dry Ice
Fluorescence
High
qPCR, RT-PCR
Cells
500 Reactions
cDNA Synthesis Kit
500 Reactions
Tube
500 Reactions
SuperMix or Master Mix
Videos
SDS
Documents
Certificates
Promotions

Promotions