Musashi-2, clone: C1, eBioscience™
Mouse Monoclonal Antibody
Marke: Affymetrix eBioscience 14-9677-80
Weitere Details : Gewicht : 0.09500kg
BeschreibungDescription: The monoclonal antibody C1 recognizes human and mouse Musashi-2 (Msi2), a highly conserved RNA-binding protein. Musashi-2 contains two RNA-recognition domains and is expressed as one of two isoforms of 36 and 37kDa. Musashi 2 with its family member Musashi-1, are expressed at high levels in stem and tumor cells. During neural development, Musashi-2 is expressed in progenitor cells in the ventricular and subventricular zones, while postnatally, Musashi-2 expression is limited to multiple subsets of neurons and astrocytes in addition to adult stem cell populations. Functionally, Musashi-2 associates with Sox2 and is required for self-renewal of embryonic stem cells. Much like its role in maintaining stemness in neural progenitors, Musashi-2 is expressed in hematopoetic stem cells and plays a similar role in maintaining self-renewal. Musashi-2 has been found to be overexpressed in human myeloid leukemia and the levels of expression correlate with decreased survival. Musashi-2 may serve as a prognostic marker for acute myeloid leukemia (AML).Applications Reported: This C1 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This C1 antibody has been tested immunohistochemistry on human FFPE tissue using high or low pH antigen retrieval at less than or equal to 10 ug/mL. The C1 antibody has also been tested by western blot on reduced whole mouse brain and SK-N-SH cell lysate and can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|MSI2H, RNA-binding protein Musashi homolog 2, musashi-2|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
|IgG3, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.