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Invitrogen™ Qtracker™ 565 Cell Labeling Kit

Designed for loading cells grown in culture with highly fluorescent Qdot nanocrystals

Brand:  Invitrogen™ Q25031MP

896.04 EUR valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.00450kg

Product Code. 10561865

  • 1344.00 € / Each
Estimated Shipment: 19-04-2024
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For Research Use Only. All usage must comply with product instructions.


Includes: 100μL Qtracker nanocrystals, Component A (2μM in 50mm borate buffer, pH 8.3) and 100μL Qtracker carrier, Component B (phosphate buffered saline, pH 7.2).

Description

Description

 

  • Use proprietary targeting peptide to deliver green-fluorescent Qdot 525 nanocrystals into cytoplasm of live cells
  • Once inside cells, Qtracker labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in population
  • Qtracker 525 label has Ex/Em (405-485/525)nm
  • Provide intense, stable fluorescence that can be traced through several generations
  • Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays
  • Cytoplasmic delivery by this mechanism is not mediated by specific enzyme; therefore, no cell-type specificity has been observed
  • Delivery is typically accomplished in less than 1 hour

 

 

  • Compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity
  • Observe labeled cells using extensive continuous illumination, without photobleaching and degradation problems often associated with organic dyes
  • Distributed in vesicles in cytoplasm, and are inherited by daughter cells for at least six generations
  • Can be seen up to a week after delivery in some cell lines
  • Do not leak out of intact cells to be taken up by adjacent cells in population
  • Qtracker reagent-labeled live cells can be easily monitored on variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems
  • Cytotoxicity of materials has been tested in variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells: Labeling appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility
  • Post-labeling, researchers have demonstrated wide variety of applications including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics

 

Cell Analysis, Cell Tracing and Tracking, Cellular Imaging, Immunofluorescence (IF)

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

Contains 100 μl Qtracker™ nanocrystals, Component A (2 μM in 50 mM borate buffer, pH 8.3) and 100 μl Qtracker™ carrier,Component B (phosphate buffered saline, pH 7.2). Store at 2–6°C. Do not freeze. Stable for at least 6 months.
Cell Labeling Reagents
Cell Labeling
QTRACKER™, Qdot™
Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.