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Invitrogen™ EnzChek™ Phospholipase A1 Assay Kit

For continuous monitoring of phospholipase A1 (PLA1) in purified enzyme preparations, cell lysates and living cells

Brand:  Invitrogen™ E10219

262.67 EUR valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.00450kg

Product Code. 10627215

  • 394.00 € / Each
Estimated Shipment: 12-04-2024
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For Research Use Only. All usage must comply with product instructions.


Includes: 1 kit; Sufficient material is supplied for 200 reactions in 96-well microplates at 100μL per well as described in the protocol below or 400 reaction in 384 low volume microplates at a volume of ≤25μL per well.

Description

Description

This kit takes the stand-alone assay for phospholipase A1, PLA1 and combines the necessary reagents to run 2 to 10 complete 96-well microplate assays that monitor activity in purified enzyme preparations and cell lysates. The importance of phospholipases in cellular signaling, lipid metabolism, inflammatory responses and pathological disorders related to these processes has stimulated demand for fluorescence-based activity monitoring methods. In particular the phospholipases resident in plasma and endothelium can perturb circulating LDL and HDL particles, creating pro-artherogenic forms. Recent evidence is drawing a link between these lipases and the progression of several severe neurodegenerative diseases, including Alzheimer's. PLA-1 improves upon two existing reagents in two ways: first it is now PLA-1 specific and secondly it has improved the assay quality by decreasing initial background noise.

Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

1 kit. Store kit at -<20°C, protected from light, desiccated

Sufficient material is supplied for 200 reactions in 96 well microplates at 100 μl per well as described in the protocol below or 400 reaction in 384 low volume microplates at a volume of ≤ 25 μL per well.
Phospholipase Assay
EnzChek™, Molecular Probes™
Room Temperature
Phospholipase Substrate
Fluorescence Intensity
Phospholipase Assay
200 reactions, 200 Reactions
Lipid-Based Substrate, Chemical Substrate
Phospholipase
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For Research Use Only. Not for use in diagnostic procedures.