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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli
Designed for cloning PCR products directly from a PCR reaction in just 5 minutes
Brand: Invitrogen™ K462040
Additional Details : Weight : 0.24000kg
Description
Uses a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants.
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
- Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
- Easy blue/white colony screening for selection of recombinants
Cloning, PCR Cloning
Specifications
| DH5α | |
| One Shot™ | |
| TOPO-TA Cloning Vectors | |
| T7, SP6 |
| TOPO™-TA | |
| 50 Reactions | |
| Cloning Kit |
For Research Use Only. Not for use in diagnostic procedures.