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IL-1 beta (Pro-form) Rat anti-Mouse, FITC, Clone: NJTEN3, eBioscience™

Rat Monoclonal Antibody

Marke:  Affymetrix eBioscience 11-7114-80

Weitere Details : Gewicht : 0.25000kg

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Artikelnummer. 15208359

  • 125.00 € / 25 Mikrogramm


Verfügbar ab
09-12-2020
Zum Warenkorb hinzufügen

Beschreibung

Beschreibung

The NJTEN3 antibody recognizes only the pro-form of mouse IL-1 beta and does not see the active (cleaved) form. Applications Reported: This NJTEN3 antibody has been reported for use in intracellular staining and flow cytometric analysis. Applications Tested: This NJTEN3 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions.IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.
Spezifikation

Spezifikation

IL-1 beta (Pro-form)
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P10749
Il1b
IgG1, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16176
Flow Cytometry
NJTEN3
FITC
Il1b
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.