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Invitrogen™ TGF beta-1 Human/Mouse Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of human or mouse TGF beta 1 protein levels from samples including serum, plasma, and cell culture supernatants. This second generation kit has increased sensitivity with a range of 8-1000pg/mL.

221.00 € - 782.00 €

Specifications

Accession Number P01137,P04202
Assay Range 8-1,000 pg/mL
Assay Sensitivity 8 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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15521227
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Invitrogen™
88-8350-76
20 x 96 Tests
782.00 €
Pack of 20
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15541227
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Invitrogen™
88-8350-86
10 x 96 Tests
544.00 €
Pack of 10
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15298897
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Invitrogen™
88-8350-22
2 x 96 Tests
221.00 €
2 sets
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Description

Description

The Human/Mouse Transforming Growth Factor beta 1 (TGFβ1) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human/mouse TGFβ1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Human/Mouse TGF beta 1 Ready-SET-Go! ELISA Set contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA)
Specifications

Specifications

P01137,P04202
8 pg/mL
ELISA Kit
Human, Mouse
Colorimetric Microplate Reader
IFN-g,Ifg
HRP
Plasma, 20 μL; Serum, 20 μL; Supernatant, 100 μL
Transforming Growth Factor beta 1, TGF-b1
1 hr. 45 min.
8-1,000 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
21803, 7040
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards., 96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22; -76; and -86)
RUO
2°C to 8°C
Human
24 hr. 30 min.
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