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Invitrogen™ TNF alpha Equine Uncoated ELISA Kit

Uncoated ELISA Kit

Brand:  Invitrogen™ ESS0017

651.70 EUR valid until 2024-03-29
Use promo code "21653" to get your promotional price.



Additional Details : Weight : 1.36080kg

Product Code. 10351154

  • 686.00 € / 1 set
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For Research Use Only. All usage must comply with product instructions.


Description

Description

The Equine TNF-alpha Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of equine TNFalpha. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states. TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.
Specifications

Specifications

P29553
<15.6 pg/mL
Biotin
ELISA Kit
No significant cross-reaction with bovine, mouse, or rat TNFa. Partial cross-reaction occurs with human and porcine TNFa (<10%)
Equine
100033834
Coating Antibody, Lyophilized Recombinant Standard, Detection Antibody, Streptavidin-HRP, Substrate Solution, Ready to use Stop Solution, 0.16M Sulfuric Acid, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Absorbance at 450nm minus absorbance at 550nm
5 x 96 Tests
Serum, 100 μL; Supernatant, 100 μL
Equine
2 hr. 50 min.
15.6-1000 pg/mL
Equine TNF alpha ELISA Reagent Kit
Colorimetric ELISA (TMB substrate) with Plates
Serum, Supernatant
5 x 96 microplate wells
Colorimetric Microplate Reader
TNF-a,TNF-alpha,TNFA,TNFSF2
HRP
Intra-assay CV <10%; inter assay CV <10%
RUO
2°C to 8°C
24 hr.
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