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Ea52-68 peptide bound to I-Ab Mouse anti-Mouse, Clone: eBioY-Ae (YAe, Y-Ae), eBioscience™

Mouse Monoclonal Antibody

Marke:  Affymetrix eBioscience 14-5741-82

Weitere Details : Gewicht : 0.01000kg

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Artikelnummer. 15207447

  • 141.00 € / 100 Mikrogramm


Verfügbar ab
05-01-2021
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Beschreibung

Beschreibung

Applications Reported: This eBioY-Ae (YAe, Y-Ae) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioY-Ae (YAe, Y-Ae) antibody has been tested by flow cytometric analysis of a mouse strain known to express the I-A(b)/MHC Class II:Ea peptide complex. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.
Spezifikation

Spezifikation

Ea52-68 peptide bound to I-Ab
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P06342
H-2Eb, H2Eb, Ia-4, Ia4, MGC163794, MGC163796
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
eBioY-Ae (YAe, Y-Ae)
Unconjugated
H2-Ab1
Liquid
H2-Ab1
IgG2b
100 μg
4° C
Primary
14961
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.