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Invitrogen™ Click-iT™ sDIBO Alkynes for copper-free click chemistry

Experience copper-free 'click chemistry' with Click-IT™ Alexa Fluor&trade, and sDIBO Alkyne reagents, which are ideal for surface labeling of live cells and minimize damage to enzymes and fluorescent proteins.

Brand:  Invitrogen™ C20023

Additional Details : Weight : 0.00910kg

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Product Code. 15950573

  • 584.00 € / 1mg
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For Research Use Only. All usage must comply with product instructions.


Description

Description

Achieve efficient labeling of azides with Click-It sDIBO Alkynes. Our Click-IT sDIBO alkynes enable the labeling of azido labeled targets without the need for copper catalysis and are available with a variety of Alexa Fluor or biotin labels, as well as amine or thiol reactive groups for creating your own conjugates. Macromolecules that have been azido-modified enzymatically, chemically, or metabolically can now be labeled easily, yielding soluble bioconjugates.

Click-iT sDIBO Alkyne labels react with azides via a copper-free Click chemistry reaction to form a stable triazole linkage with an Alexa Fluor, Biotin, or label of your choice. Copper-free click reactions using our sDIBO alkynes are ideal for surface labeling of live cells; they also minimize damage to fluorescent proteins like GFP or R-PE. For intracellular labeling, the nonspecific binding of DIBO alkynes creates a background signal, and a labeling strategy using picolyl azides is the preferred solution in these instances.

Click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be labeled more easily, yielding more soluble bioconjugates with improved biological labeling utility.

To make a concentrated stock solution from 2–5 mM, dissolve the dye-alkyne in high quality anhydrous DMSO. Store frozen, desiccated, and protected from light. Click-iT SDP Ester sDIBO alkyne is susceptible to hydrolysis, so prepare the stock solution fresh on the day of the experiment.

Specifications

Specifications

Less hazardous
∼1000
Click Chemistry
sDIBO Alkyne
Click-iT™
Contains 1 mg of Biotin sDIBO Alkyne
Store at ≤ -20°C. Desiccation recommended. Protect material from long-term exposure to light; may be exposed to light for short periods of time
Dry Ice
Biotin
Azide
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