Learn More
CD8a Monoclonal Antibody (C8/144B), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0085-80
Additional Details : Weight : 0.01000kg
Description
Description: The monoclonal antibody C8/144B recognizes the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. Applications Reported: This C8/144B antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This C8/144B antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue using IHC Antigen Retrieval Solution - High pH (cat. 00-4956). This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
CD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).Specifications
CD8a | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P01732 | |
CD8A | |
Affinity chromatography | |
RUO | |
925 | |
4° C | |
Liquid |
Immunohistochemistry (Paraffin) | |
C8/144B | |
Unconjugated | |
CD8A | |
CD8, Leu-2, MAL, p32, CD8a | |
Mouse | |
25 μg | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
For Research Use Only.