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CD54 (ICAM-1) Monoclonal Antibody (HA58), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

53.75 € - 118.00 €

Specifications

Antigen CD54 (ICAM-1)
Clone HA58
Concentration 0.5 mg/mL
Applications Flow Cytometry, Functional Assay, Immunohistochemistry, Neutralization, Western Blot
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15247027
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Affymetrix eBioscience
14-0549-82
100 μg
118.00 €
100µg
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15237027
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Affymetrix eBioscience
14-0549-80
25 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response. Applications Reported: This HA58 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. HA58 has also been reported in blocking of in vitro mixed lymphocyte reaction. (Please use Functional Grade purified HA58, cat. 16-0549, in functional assays.). Applications Tested: The HA58 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
Specifications

Specifications

CD54 (ICAM-1)
0.5 mg/mL
Monoclonal
Liquid
RUO
P05362
ICAM1
Primary
4° C
ICAM1
HA58
Flow Cytometry, Functional Assay, Immunohistochemistry, Neutralization, Western Blot
Unconjugated
Mouse
Human
3383
IgG1 κ
Affinity chromatography
Antibody
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