CD40, clone: 5C3, eBioscience™
Mouse Monoclonal Antibody
Marke: Affymetrix eBioscience 14-0409-80
Weitere Details : Gewicht : 0.01000kg
BeschreibungDescription: The 5C3 monoclonal antibody reacts with human CD40, a 45-50kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.Applications Reported: The 5C3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 5C3 has also been reported in in vitro functional studies. (Please use Functional Grade purified 5C3, cat. 16-0409, in functional assays.).Applications Tested: The 5C3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Bp50, CDW40, MGC9013, TNFRSF5, p50|
|Flow CytometryþImmunohistochemistry (Frozen)|
|IgG1, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.