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CD34 Monoclonal Antibody (RAM34), eFluor™ 660, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

117.00 € - 246.00 €

Specifications

Antigen CD34
Clone RAM34
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15371860
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Affymetrix eBioscience
50-0341-80
25 μg
117.00 €
25µg
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15381860
View Documents Promotion Details
Affymetrix eBioscience
50-0341-82
100 μg
246.00 €
100µg
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Description

Description

Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers. Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

CD34 Monoclonal antibody specifically detects CD34 in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

CD34
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
(E3-independent) E2 ubiquitin-conjugating enzyme R1; AU040960; CD34; CD34 antigen; CD34 molecule; CDC34; cell division cycle 34; cell division cycle 34 homolog; cluster designation 34; cluster of differentiation antigen 34; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell marker CD34; Mucosialin; RP11-328D5.2; sialomucin protein; type 1 transmembrane protein; UBC3; UBCH3; UBE2R1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
Cd34
Primary
4° C, store in dark, DO NOT FREEZE!
Cd34
RAM34
Flow Cytometry
eFluor 660
Rat
Mouse
Q64314
12490
IgG2a κ
Affinity chromatography
Antibody
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