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CD3, clone: UCHT1, eBioscience™

Mouse Monoclonal Antibody

Marke:  Affymetrix eBioscience 14-0038-82

Weitere Details : Gewicht : 0.01000kg

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Artikelnummer. 15296737

  • 85.75 € / 100 Mikrogramm


Auf Lager: 16
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Beschreibung

Beschreibung

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: The UCHT1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. UCHT1 has also been reported in activation of T cells. (Please use Functional Grade purified UCHT1, cat. 16-0038, in functional assays.).Applications Tested: The UCHT1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Spezifikation

Spezifikation

CD3
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P09693, P20963, P04234, P07766
FLJ18683, T3E, TCRE
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen)
UCHT1
Unconjugated
CD3E
Liquid
CD247, CD3D, CD3E, CD3G
IgG1, kappa, kappa
100μg
4° C
Primary
915, 916, 917, 919
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