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CD28 Monoclonal Antibody (37.51), Functional Grade, eBioscience™, Invitrogen™

Syrian Hamster Monoclonal Antibody

99.25 € - 532.00 €

Specifications

Antigen CD28
Clone 37.51
Concentration 1 mg/mL
Applications Flow Cytometry, Functional Assay
Classification Monoclonal
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Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15218417
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Affymetrix eBioscience
16-0281-81
50 μg
99.25 €
50µg
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15228417
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Affymetrix eBioscience
16-0281-82
100 μg
165.00 €
100µg
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15238417
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Affymetrix eBioscience
16-0281-85
500 μg
281.00 €
500µg
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15248417
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Affymetrix eBioscience
16-0281-86
1 mg
532.00 €
1mg
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Description

Description

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis. 37.51 has also been reported in costimulation of T cells in vitro and in vivo . Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
Specifications

Specifications

CD28
1 mg/mL
Monoclonal
Liquid
RUO
PBS with no preservative; pH 7.2
antigen CD28; CD28; CD28 antigen; CD28 antigen (Tp44); CD28 isoform; CD28 isoform 2; Cd28 molecule; CD28 precursor protein; CD28 protein; CD28 protein precursor; CD28RNA; cell surface protein; CHT28; contains partial extracellular domain; costimulatory molecule B7 receptor CD28; EGK_04709; MGC138290; sCD28; soluble CD28; T44; T-cell costimulatory molecule CD28; T-cell costimulatory molecule Tp44; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28 homolog; TP44
Cd28
Primary
4° C
Cd28
37.51
Flow Cytometry, Functional Assay
Functional Grade
Syrian Hamster
Mouse
P31041
12487
IgG
Affinity chromatography
Antibody
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