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alpha-Fetoprotein Monoclonal Antibody (AFP3), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-6583-80

Additional Details : Weight : 0.01000kg

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Product Code. 15237727

  • 198.00 € / 25µg
Estimated Shipment: 06-05-2024
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Description

Description

Description: This AFP3 monoclonal antibody reacts with human alpha-fetoprotein (AFP). This 70-kDa secretory protein is a member of the albumin gene family. Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum. After birth, serum AFP levels decrease dramatically. In fact, AFP is nearly undetectable in normal adult serum. However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults. As such, detection of this protein is frequently used as a diagnostic marker for these conditions. When performing western blotting or immunohistochemistry on paraffin section, we recommend the use of monoclonal antibody 1E8 (cat. 14-9499). Applications Reported: This AFP3 antibody has been reported for use in immunoblotting (WB) and immunocytochemistry. Applications Tested: This AFP3 antibody has been tested by western blot analysis of reduced HepG2 cell lysate, as well as by immunocytochemistry of HepG2 cells. For western blotting, this antibody can be used at 1-10 μg/mL. (Western blotting of non-reduced cell lysate yields a band at approximately 58 kDa.) For immunocytochemistry, AFP3 can be used at 1-5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.

AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric, dimeric, and trimeric forms. AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
Specifications

Specifications

alpha-Fetoprotein
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P02771
AFP
Affinity chromatography
RUO
174
4° C
Liquid
Immunocytochemistry, Western Blot
AFP3
Unconjugated
AFP
a fetoglobulin; a fetoprotein; Afp; AFPD; Alpha fetoglobulin; alpha fetoprotein; alpha-1-fetoprotein; Alpha-fetoglobulin; alpha-fetoprotein; alpha-fetoprotein precursor; alpha-foetoprotein; chloride intracellular channel 6; FETA; fetoglobulin; fetoprotein; HPAFP; a fetoglobulin; a fetoprotein
Mouse
25 μg
Primary
Human
Antibody
IgG1 κ
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